Part:BBa_K883050:Design

CCMV 25 AA N terminal deletion mutant

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 68
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We had to determine the starting point of our construct, and add an ATG in the primer to ensure the start of the translation

Source

We got this template in a pET28 plasmid from the Virology group Wageningen UR (Dr. Kormelink)